BMRB Entry 10001
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR10001
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Title: A high resolution structure of mastoparan-X strongly bound to lipid-bilayer membrane determined by solid-state NMR PubMed: 16714348
Deposition date: 2005-07-31 Original release date: 2006-06-28
Authors: Akutsu, Hideo; Fujiwara, Toshimichi; Todokoro, Yasuto
Citation: Todokoro, Yasuto; Yumen, Ikuko; Fukushima, Kei; Kang, S.-W.; Park, J.-S.; Kohno, Toshiyuki; Wakamatsu, Kaori; Akutsu, Hideo; Fujiwara, Toshimichi. "Structure of Tightly Membrane-Bound Mastoparan-X, a G-protein-Activating Peptide, Determined by Solid-State NMR" Biophys. J. 91, 1368-1379 (2006).
Assembly members:
MP-X, polymer, 14 residues, 1556 Da.
Natural source: Common Name: wasps Taxonomy ID: 7448 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Vespa simillima
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MP-X: INWKGIAAMAKKLX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 73 |
| 15N chemical shifts | 20 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MP-X | 1 |
Entities:
Entity 1, MP-X 14 residues - 1556 Da.
| 1 | ILE | ASN | TRP | LYS | GLY | ILE | ALA | ALA | MET | ALA | ||||
| 2 | LYS | LYS | LEU | NLW |
Samples:
sample_1: mastoparan-X, [U-95% 13C; U-95% 15N], 5 mg; liposome 50 mg
condition_1: pH: 7.4; temperature: 253 K
condition_2: pH: 7.4; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D N-CA-CO | sample_1 | isotropic | condition_1 |
| 3D Ni-COi+1-CAi+1 | sample_1 | isotropic | condition_1 |
| 2D RFDR | sample_1 | isotropic | condition_1 |
| 2D SPCz5 | sample_1 | isotropic | condition_1 |
| 2D (CACB)i+1-(COCA)i | sample_1 | isotropic | condition_2 |
| 2D Ni-(COCA)i+1 | sample_1 | isotropic | condition_2 |
| 2D N-CA | sample_1 | isotropic | condition_2 |
| 2D Ni-(COCACB)i+1 | sample_1 | isotropic | condition_1 |
Software:
Felix2000 v2000, Accelrys Inc. - assignment
spinsight v4.3.2, VARIAN - processing
NMR spectrometers:
- Varian CMX 600 MHz
- Varian CMX 500 MHz