BMRB Entry 10024
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR10024
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Title: Chemical shift assignments for the Rhodobacter sphaeroides PufX membrane protein PubMed: 17335288
Deposition date: 2006-08-03 Original release date: 2007-06-13
Authors: Wang, Zheng-Yu; Suzuki, H.; Kudo, M.; Kobayashi, M.; Nozawa, T.
Citation: Wang, Zheng-Yu; Suzuki, H.; Kobayashi, M.; Nozawa, T.. "Solution structure of the Rhodobacter sphaeroides PufX membrane protein: implications for the quinone exchange and protein-protein interactions." Biochemistry 46, 3635-3642 (2007).
Assembly members:
PufX polypeptide, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: Rhodobacter sphaeroides Taxonomy ID: 1063 Superkingdom: bacteria Kingdom: Not applicable Genus/species: Rhodobacter sphaeroides
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PufX polypeptide: ADKTIFNDHLNTNPKTNLRL
WVAFQMMKGAGWAGGVFFGT
LLLIGFFRVVGRMLPIQENQ
APAPNITGALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 133 |
| 15N chemical shifts | 74 |
| 1H chemical shifts | 352 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 77 residues - Formula weight is not available
| 1 | ALA | ASP | LYS | THR | ILE | PHE | ASN | ASP | HIS | LEU | ||||
| 2 | ASN | THR | ASN | PRO | LYS | THR | ASN | LEU | ARG | LEU | ||||
| 3 | TRP | VAL | ALA | PHE | GLN | MET | MET | LYS | GLY | ALA | ||||
| 4 | GLY | TRP | ALA | GLY | GLY | VAL | PHE | PHE | GLY | THR | ||||
| 5 | LEU | LEU | LEU | ILE | GLY | PHE | PHE | ARG | VAL | VAL | ||||
| 6 | GLY | ARG | MET | LEU | PRO | ILE | GLN | GLU | ASN | GLN | ||||
| 7 | ALA | PRO | ALA | PRO | ASN | ILE | THR | GLY | ALA | LEU | ||||
| 8 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: PufX polypeptide, [U-13C; U-15N], 2 mM; Chloroform 50%; Methanol 50%
condition_1: temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC-TOCSY | sample_1 | not available | condition_1 |
| HSQC-NOESY | sample_1 | not available | condition_1 |
| HNCA | sample_1 | not available | condition_1 |
| HN(CO)CA | sample_1 | not available | condition_1 |
| CBCANH | sample_1 | not available | condition_1 |
| CBCA(CO)NH | sample_1 | not available | condition_1 |
| HNHA | sample_1 | not available | condition_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance DRX400 400 MHz
Related Database Links:
| PDB | |
| EMBL | CAB38756 |
| GB | AAA26129 AAF24306 ABA79427 ABN77002 ACM01440 |
| REF | WP_002720419 YP_353328 |
| SP | P13402 |
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