BMRB Entry 10046
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10046
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Title: Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)
Deposition date: 2006-11-09 Original release date: 2008-08-13
Authors: Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)" . ., .-..
Assembly members:
PB1 domain, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PB1 domain: GSSGSSGPFCAMENQVLVIR
IKIPNSGAVDWTVHSGPQLL
FRDVLDVIGQVLPEATTTAF
EYEDEDGDRITVRSDEEMKA
MLSYYYSTVMEQQVNGQLIE
PLQIFPRSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 473 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 743 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mitogen activated protein kinase kinase 5 | 1 |
Entities:
Entity 1, Mitogen activated protein kinase kinase 5 113 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | PHE | CYS | ||||
| 2 | ALA | MET | GLU | ASN | GLN | VAL | LEU | VAL | ILE | ARG | ||||
| 3 | ILE | LYS | ILE | PRO | ASN | SER | GLY | ALA | VAL | ASP | ||||
| 4 | TRP | THR | VAL | HIS | SER | GLY | PRO | GLN | LEU | LEU | ||||
| 5 | PHE | ARG | ASP | VAL | LEU | ASP | VAL | ILE | GLY | GLN | ||||
| 6 | VAL | LEU | PRO | GLU | ALA | THR | THR | THR | ALA | PHE | ||||
| 7 | GLU | TYR | GLU | ASP | GLU | ASP | GLY | ASP | ARG | ILE | ||||
| 8 | THR | VAL | ARG | SER | ASP | GLU | GLU | MET | LYS | ALA | ||||
| 9 | MET | LEU | SER | TYR | TYR | TYR | SER | THR | VAL | MET | ||||
| 10 | GLU | GLN | GLN | VAL | ASN | GLY | GLN | LEU | ILE | GLU | ||||
| 11 | PRO | LEU | GLN | ILE | PHE | PRO | ARG | SER | GLY | PRO | ||||
| 12 | SER | SER | GLY |
Samples:
sample_1: PB1 domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 297 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
VNMR v6.1c, Varian - collection
NMRView v5.0.4, Jhonson, B.A. - data analysis
Kujira v0.896, Kobayashi, N. - data analysis
CYANA v1.0.8, Guntert, P. - refinement, structure solution
NMRPipe v20020425, Delaglio, F. - processing
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts