BMRB Entry 10058
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10058
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Title: Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein
Deposition date: 2006-11-29 Original release date: 2008-08-13
Authors: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein" . ., .-..
Assembly members:
PICOT homology 2 domain, polymer, 109 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PICOT homology 2 domain: GSSGSSGLKVLTNKASVMLF
MKGNKQEAKCGFSKQILEIL
NSTGVEYETFDILEDEEVRQ
GLKTFSNWPTYPQLYVRGDL
VGGLDIVKELKDNGELLPIL
KGESGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 452 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 724 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Thioredoxin-like protein 2 | 1 |
Entities:
Entity 1, Thioredoxin-like protein 2 109 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LEU | LYS | VAL | ||||
| 2 | LEU | THR | ASN | LYS | ALA | SER | VAL | MET | LEU | PHE | ||||
| 3 | MET | LYS | GLY | ASN | LYS | GLN | GLU | ALA | LYS | CYS | ||||
| 4 | GLY | PHE | SER | LYS | GLN | ILE | LEU | GLU | ILE | LEU | ||||
| 5 | ASN | SER | THR | GLY | VAL | GLU | TYR | GLU | THR | PHE | ||||
| 6 | ASP | ILE | LEU | GLU | ASP | GLU | GLU | VAL | ARG | GLN | ||||
| 7 | GLY | LEU | LYS | THR | PHE | SER | ASN | TRP | PRO | THR | ||||
| 8 | TYR | PRO | GLN | LEU | TYR | VAL | ARG | GLY | ASP | LEU | ||||
| 9 | VAL | GLY | GLY | LEU | ASP | ILE | VAL | LYS | GLU | LEU | ||||
| 10 | LYS | ASP | ASN | GLY | GLU | LEU | LEU | PRO | ILE | LEU | ||||
| 11 | LYS | GLY | GLU | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PICOT homology 2 domain, [U-13C; U-15N], 0.65 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB |
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