BMRB Entry 10071

Name: Solution structure of RRM domain in RNA-binding protein NP_057951
Published: 2008-08-14

Click here to enlarge.

PDB ID: 1wf1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10071
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution structure of RRM domain in RNA-binding protein NP_057951

Deposition date: 2006-12-25 Original release date: 2008-08-14

Authors: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of RRM domain in RNA-binding protein NP_057951" . ., .-..

Assembly members:
RRM domain, polymer, 110 residues, Formula weight is not available

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: cell free synthesis

Entity Sequences (FASTA):
RRM domain: GSSGSSGMSLKLQASNVTNK NDPKSINSRVFIGNLNTALV KKSDVETIFSKYGRVAGCSV HKGYAFVQYSNERHARAAVL GENGRVLAGQTLDINMAGEP KPDRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	442
15N chemical shifts	108
1H chemical shifts	701

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA-binding protein Raly	1

Entities:

Entity 1, RNA-binding protein Raly 110 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	MET	SER	LEU
2	LYS	LEU	GLN	ALA	SER	ASN	VAL	THR	ASN	LYS
3	ASN	ASP	PRO	LYS	SER	ILE	ASN	SER	ARG	VAL
4	PHE	ILE	GLY	ASN	LEU	ASN	THR	ALA	LEU	VAL
5	LYS	LYS	SER	ASP	VAL	GLU	THR	ILE	PHE	SER
6	LYS	TYR	GLY	ARG	VAL	ALA	GLY	CYS	SER	VAL
7	HIS	LYS	GLY	TYR	ALA	PHE	VAL	GLN	TYR	SER
8	ASN	GLU	ARG	HIS	ALA	ARG	ALA	ALA	VAL	LEU
9	GLY	GLU	ASN	GLY	ARG	VAL	LEU	ALA	GLY	GLN
10	THR	LEU	ASP	ILE	ASN	MET	ALA	GLY	GLU	PRO
11	LYS	PRO	ASP	ARG	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: RRM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl(pH 7.0) 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	condition_1
3D 13C-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WF1
DBJ	BAD96635 BAF83743 BAF83768 BAG61653 BAI46166
GB	AAC28898 AAF04487 AAI03754 AAI05019 ACA53514
REF	NP_001267354 NP_031393 NP_057951 XP_001102633 XP_002747313
SP	Q9UKM9

Biological Magnetic Resonance Data Bank