BMRB Entry 10090
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10090
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Title: Solution structure of hypothetical protein F20O9.120 from Arabidopsis thaliana
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Sato, M.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of hypothetical protein F20O9.120 from Arabidopsis thaliana" . ., .-..
Assembly members:
residues 14-145, polymer, 145 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
residues 14-145: GSSGSSGSTVKRKPVFVKVE
QLKPGTTGHTLTVKVIEANI
VVPVTRKTRPASSLSRPSQP
SRIVECLIGDETGCILFTAR
NDQVDLMKPGATVILRNSRI
DMFKGTMRLGVDKWGRIEAT
GAASFTVKEDNNLSLVEYES
GPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 569 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 952 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hypothetical protein F20O9.120 | 1 |
Entities:
Entity 1, hypothetical protein F20O9.120 145 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | THR | VAL | ||||
| 2 | LYS | ARG | LYS | PRO | VAL | PHE | VAL | LYS | VAL | GLU | ||||
| 3 | GLN | LEU | LYS | PRO | GLY | THR | THR | GLY | HIS | THR | ||||
| 4 | LEU | THR | VAL | LYS | VAL | ILE | GLU | ALA | ASN | ILE | ||||
| 5 | VAL | VAL | PRO | VAL | THR | ARG | LYS | THR | ARG | PRO | ||||
| 6 | ALA | SER | SER | LEU | SER | ARG | PRO | SER | GLN | PRO | ||||
| 7 | SER | ARG | ILE | VAL | GLU | CYS | LEU | ILE | GLY | ASP | ||||
| 8 | GLU | THR | GLY | CYS | ILE | LEU | PHE | THR | ALA | ARG | ||||
| 9 | ASN | ASP | GLN | VAL | ASP | LEU | MET | LYS | PRO | GLY | ||||
| 10 | ALA | THR | VAL | ILE | LEU | ARG | ASN | SER | ARG | ILE | ||||
| 11 | ASP | MET | PHE | LYS | GLY | THR | MET | ARG | LEU | GLY | ||||
| 12 | VAL | ASP | LYS | TRP | GLY | ARG | ILE | GLU | ALA | THR | ||||
| 13 | GLY | ALA | ALA | SER | PHE | THR | VAL | LYS | GLU | ASP | ||||
| 14 | ASN | ASN | LEU | SER | LEU | VAL | GLU | TYR | GLU | SER | ||||
| 15 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: residues 14-145, [U-13C; U-15N], 0.8 mM; d-Tris HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAA16883 CAB79646 |
| GB | AAK93582 AAL08288 AAM14346 AAM65628 AEE85487 |
| REF | NP_194573 XP_002869502 |
| SP | O49453 |
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