BMRB Entry 10095
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10095
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Title: Solution structure of the KH domain of human ribosomal protein S3
Deposition date: 2007-02-13 Original release date: 2008-08-29
Authors: Nameki, N.; Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation: Nameki, N.; Tomizawa, T.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the KH domain of human ribosomal protein S3" Not known ., .-..
Assembly members:
40S ribosomal protein S3, polymer, 92 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
40S ribosomal protein S3: GSSGSSGFKAELNEFLTREL
AEDGYSGVEVRVTPTRTEII
ILATRTQNVLGEKGRRIREL
TAVVQKRFGFPEGSVELYAE
KVATRGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 362 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 572 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 40S ribosomal protein S3 | 1 |
Entities:
Entity 1, 40S ribosomal protein S3 92 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PHE | LYS | ALA | ||||
| 2 | GLU | LEU | ASN | GLU | PHE | LEU | THR | ARG | GLU | LEU | ||||
| 3 | ALA | GLU | ASP | GLY | TYR | SER | GLY | VAL | GLU | VAL | ||||
| 4 | ARG | VAL | THR | PRO | THR | ARG | THR | GLU | ILE | ILE | ||||
| 5 | ILE | LEU | ALA | THR | ARG | THR | GLN | ASN | VAL | LEU | ||||
| 6 | GLY | GLU | LYS | GLY | ARG | ARG | ILE | ARG | GLU | LEU | ||||
| 7 | THR | ALA | VAL | VAL | GLN | LYS | ARG | PHE | GLY | PHE | ||||
| 8 | PRO | GLU | GLY | SER | VAL | GLU | LEU | TYR | ALA | GLU | ||||
| 9 | LYS | VAL | ALA | THR | ARG | GLY | SER | GLY | PRO | SER | ||||
| 10 | SER | GLY |
Samples:
sample_1: KH domain, [U-13C; U-15N], 0.6 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.901, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - structure solution
OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22624 BAB27042 BAB27761 BAB28111 BAB28159 |
| EMBL | CAA35916 CAA39248 CAA40592 CAA54167 CAA84290 |
| GB | AAA18095 AAB19349 AAB46849 AAB60336 AAB60337 |
| REF | NP_000996 NP_001009239 NP_001026007 NP_001029219 NP_001038066 |
| SP | E2RH47 P02350 P23396 P47835 P62908 |
| TPG | DAA21909 |
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