BMRB Entry 10114
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10114
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Title: Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse
Deposition date: 2007-02-15 Original release date: 2008-08-15
Authors: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the C-terminal Pleckstrin Homology Domain of Sbf1 from Mouse" . ., .-..
Assembly members:
The Pleckstrin Homology Domain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
The Pleckstrin Homology Domain: GSSGSSGRSYEGILYKKGAF
MKPWKARWFVLDKTKHQLRY
YDHRMDTECKGVIDLAEVEA
VAPGTPTIGAPKTVDEKAFF
DVKTTRRVYNFCAQDVPSAQ
QWVDRIQSCLSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 520 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 785 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SET binding factor 1 | 1 |
Entities:
Entity 1, SET binding factor 1 117 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | SER | TYR | ||||
| 2 | GLU | GLY | ILE | LEU | TYR | LYS | LYS | GLY | ALA | PHE | ||||
| 3 | MET | LYS | PRO | TRP | LYS | ALA | ARG | TRP | PHE | VAL | ||||
| 4 | LEU | ASP | LYS | THR | LYS | HIS | GLN | LEU | ARG | TYR | ||||
| 5 | TYR | ASP | HIS | ARG | MET | ASP | THR | GLU | CYS | LYS | ||||
| 6 | GLY | VAL | ILE | ASP | LEU | ALA | GLU | VAL | GLU | ALA | ||||
| 7 | VAL | ALA | PRO | GLY | THR | PRO | THR | ILE | GLY | ALA | ||||
| 8 | PRO | LYS | THR | VAL | ASP | GLU | LYS | ALA | PHE | PHE | ||||
| 9 | ASP | VAL | LYS | THR | THR | ARG | ARG | VAL | TYR | ASN | ||||
| 10 | PHE | CYS | ALA | GLN | ASP | VAL | PRO | SER | ALA | GLN | ||||
| 11 | GLN | TRP | VAL | ASP | ARG | ILE | GLN | SER | CYS | LEU | ||||
| 12 | SER | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Pleckstrin Homology domain, [U-13C; U-15N], 0.28 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.854, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB |
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