BMRB Entry 10122
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10122
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Title: Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Nameki, N.; Koshiba, S.; Tochio, N.; Kobayashi, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Nameki, N.; Koshiba, S.; Tochio, N.; Kobayashi, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second SH3 domain of human intersectin 2 (KIAA1256)" Not known ., .-..
Assembly members:
SH3 domain, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SH3 domain: GSSGSSGVENLKAQALCSWT
AKKDNHLNFSKHDIITVLEQ
QENWWFGEVHGGRGWFPKSY
VKIIPGSESGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 313 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 482 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Intersectin 2 | 1 |
Entities:
Entity 1, Intersectin 2 74 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | VAL | GLU | ASN | ||||
| 2 | LEU | LYS | ALA | GLN | ALA | LEU | CYS | SER | TRP | THR | ||||
| 3 | ALA | LYS | LYS | ASP | ASN | HIS | LEU | ASN | PHE | SER | ||||
| 4 | LYS | HIS | ASP | ILE | ILE | THR | VAL | LEU | GLU | GLN | ||||
| 5 | GLN | GLU | ASN | TRP | TRP | PHE | GLY | GLU | VAL | HIS | ||||
| 6 | GLY | GLY | ARG | GLY | TRP | PHE | PRO | LYS | SER | TYR | ||||
| 7 | VAL | LYS | ILE | ILE | PRO | GLY | SER | GLU | SER | GLY | ||||
| 8 | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.5 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.771, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - structure solution
OPALp, Guntert, P. - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts