BMRB Entry 10123
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10123
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Title: Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3 PubMed: 15689505
Deposition date: 2007-04-02 Original release date: 2008-07-16
Authors: Nameki, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.
Citation: Nameki, N.; Tochio, N.; Koshiba, S.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Fujikura, Y.; Saito, M.; Ikari, M.; Watanabe, M.; Terada, T.; Shirouzu, M.; Yoshida, M.; Hirota, H.; Tanaka, A.; Hayashizaki, Y.; Guntert, P.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PWWP domain of the hepatoma-derived growth factor family." Protein Sci. 14, 756-764 (2005).
Assembly members:
PWWP domain, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
PWWP domain: GSSGSSGEYKAGDLVFAKMK
GYPHWPARIDELPEGAVKPP
ANKYPIFFFGTHETAFLGPK
DLFPYKEYKDKFGKSNKRKG
FNEGLWEIENSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 438 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 644 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PWWP domain | 1 |
Entities:
Entity 1, PWWP domain 96 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | TYR | LYS | ||||
| 2 | ALA | GLY | ASP | LEU | VAL | PHE | ALA | LYS | MET | LYS | ||||
| 3 | GLY | TYR | PRO | HIS | TRP | PRO | ALA | ARG | ILE | ASP | ||||
| 4 | GLU | LEU | PRO | GLU | GLY | ALA | VAL | LYS | PRO | PRO | ||||
| 5 | ALA | ASN | LYS | TYR | PRO | ILE | PHE | PHE | PHE | GLY | ||||
| 6 | THR | HIS | GLU | THR | ALA | PHE | LEU | GLY | PRO | LYS | ||||
| 7 | ASP | LEU | PHE | PRO | TYR | LYS | GLU | TYR | LYS | ASP | ||||
| 8 | LYS | PHE | GLY | LYS | SER | ASN | LYS | ARG | LYS | GLY | ||||
| 9 | PHE | ASN | GLU | GLY | LEU | TRP | GLU | ILE | GLU | ASN | ||||
| 10 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PWWP domain, [U-13C; U-15N], 0.7 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.613, Kobayashi, N. - data analysis
CYANA v1.0.6, Guntert, P. - structure solution
OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA90477 BAA90478 BAA91597 BAB31754 BAC32123 |
| GB | AAD34137 AAH15483 AAH55734 AAI51803 AAK72965 |
| REF | NP_001178297 NP_001297902 NP_038914 NP_057157 NP_665728 |
| SP | Q923W4 Q9JMG7 Q9Y3E1 |
| TPG | DAA17616 |
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