BMRB Entry 10130
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10130
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Title: Solution structure of a novel beta-grasp fold like domain of Hypothetical protein (Arabidopsis thaliana)
Deposition date: 2007-04-02 Original release date: 2008-09-02
Authors: Qin, X.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of a novel beta-grasp fold like domain of Hypothetical protein (Arabidopsis thaliana)" Not known ., .-..
Assembly members:
Hypothetical domain, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Hypothetical domain: GSSGSSGTMLRVRSRDGLER
VSVDGPHITVSQLKTLIQDQ
LQIPIHNQTLSTNRNLLLAK
SPSDFLAFTDMADPNLRISS
LNLAHGSMVYLAYEGERTIR
GSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 457 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 737 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NPL4 family protein | 1 |
Entities:
Entity 1, NPL4 family protein 107 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | MET | LEU | ||||
| 2 | ARG | VAL | ARG | SER | ARG | ASP | GLY | LEU | GLU | ARG | ||||
| 3 | VAL | SER | VAL | ASP | GLY | PRO | HIS | ILE | THR | VAL | ||||
| 4 | SER | GLN | LEU | LYS | THR | LEU | ILE | GLN | ASP | GLN | ||||
| 5 | LEU | GLN | ILE | PRO | ILE | HIS | ASN | GLN | THR | LEU | ||||
| 6 | SER | THR | ASN | ARG | ASN | LEU | LEU | LEU | ALA | LYS | ||||
| 7 | SER | PRO | SER | ASP | PHE | LEU | ALA | PHE | THR | ASP | ||||
| 8 | MET | ALA | ASP | PRO | ASN | LEU | ARG | ILE | SER | SER | ||||
| 9 | LEU | ASN | LEU | ALA | HIS | GLY | SER | MET | VAL | TYR | ||||
| 10 | LEU | ALA | TYR | GLU | GLY | GLU | ARG | THR | ILE | ARG | ||||
| 11 | GLY | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Hypothetical domain, [U-13C; U-15], 1.07 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.854, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAB87745 |
| GB | AAK59569 AAL07248 ABG89129 AEE80422 |
| REF | NP_191859 |
| SP | Q9LYC2 |
Download simulated HSQC data in one of the following formats:
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