BMRB Entry 10136
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10136
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Title: Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)
Deposition date: 2007-05-14 Original release date: 2008-09-26
Authors: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.
Citation: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)" Not known ., .-..
Assembly members:
Intersectin 2, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Intersectin 2: GSSGSSGYRALYPFEARNHD
EMSFNSGDIIQVDEKTVGEP
GWLYGSFQGNFGWFPCNYVE
KMPSSENEKAVSPKKALLPP
TVSLSATSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 392 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 576 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Intersectin 2 | 1 |
Entities:
Entity 1, Intersectin 2 93 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | ARG | ALA | ||||
| 2 | LEU | TYR | PRO | PHE | GLU | ALA | ARG | ASN | HIS | ASP | ||||
| 3 | GLU | MET | SER | PHE | ASN | SER | GLY | ASP | ILE | ILE | ||||
| 4 | GLN | VAL | ASP | GLU | LYS | THR | VAL | GLY | GLU | PRO | ||||
| 5 | GLY | TRP | LEU | TYR | GLY | SER | PHE | GLN | GLY | ASN | ||||
| 6 | PHE | GLY | TRP | PHE | PRO | CYS | ASN | TYR | VAL | GLU | ||||
| 7 | LYS | MET | PRO | SER | SER | GLU | ASN | GLU | LYS | ALA | ||||
| 8 | VAL | SER | PRO | LYS | LYS | ALA | LEU | LEU | PRO | PRO | ||||
| 9 | THR | VAL | SER | LEU | SER | ALA | THR | SER | GLY | PRO | ||||
| 10 | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 0.54 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.816, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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