BMRB Entry 10147
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10147
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the CH domain from human Sperm flagellar protein 1
Deposition date: 2007-12-04 Original release date: 2008-12-10
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CH domain from human Sperm flagellar protein 1" Not known ., .-..
Assembly members:
CH domain, polymer, 127 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
CH domain: GSSGSSGMASSVDEEALHQL
YLWVDNIPLSRPKRNLSRDF
SDGVLVAEVIKFYFPKMVEM
HNYVPANSLQQKLSNWGHLN
RKVLKRLNFSVPDDVMRKIA
QCAPGVVELVLIPLRQRLEE
RQRRRKQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 557 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 914 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sperm flagellar protein 1 | 1 |
Entities:
Entity 1, Sperm flagellar protein 1 127 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | ALA | SER | ||||
| 2 | SER | VAL | ASP | GLU | GLU | ALA | LEU | HIS | GLN | LEU | ||||
| 3 | TYR | LEU | TRP | VAL | ASP | ASN | ILE | PRO | LEU | SER | ||||
| 4 | ARG | PRO | LYS | ARG | ASN | LEU | SER | ARG | ASP | PHE | ||||
| 5 | SER | ASP | GLY | VAL | LEU | VAL | ALA | GLU | VAL | ILE | ||||
| 6 | LYS | PHE | TYR | PHE | PRO | LYS | MET | VAL | GLU | MET | ||||
| 7 | HIS | ASN | TYR | VAL | PRO | ALA | ASN | SER | LEU | GLN | ||||
| 8 | GLN | LYS | LEU | SER | ASN | TRP | GLY | HIS | LEU | ASN | ||||
| 9 | ARG | LYS | VAL | LEU | LYS | ARG | LEU | ASN | PHE | SER | ||||
| 10 | VAL | PRO | ASP | ASP | VAL | MET | ARG | LYS | ILE | ALA | ||||
| 11 | GLN | CYS | ALA | PRO | GLY | VAL | VAL | GLU | LEU | VAL | ||||
| 12 | LEU | ILE | PRO | LEU | ARG | GLN | ARG | LEU | GLU | GLU | ||||
| 13 | ARG | GLN | ARG | ARG | ARG | LYS | GLN |
Samples:
sample_1: CH domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20060524, Delaglio, F - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9823, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAG36504 |
| EMBL | CAB45745 |
| GB | AAH22476 ABQ59055 ADZ15948 AIC59905 EAX10503 |
| REF | NP_001181610 NP_056232 XP_001161874 XP_003821360 XP_003905065 |
| SP | Q9Y4P9 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts