BMRB Entry 10150
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10150
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Title: Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein
Deposition date: 2007-12-11 Original release date: 2008-12-11
Authors: Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SANT domain of fission yeast SPCC24B10.08c protein" . ., .-..
Assembly members:
SPCC24B10.08c protein, polymer, 58 residues, Formula weight is not available
Natural source: Common Name: Fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SPCC24B10.08c protein: GSSGSSGFDENWGADEELLL
IDACETLGLGNWADIADYVG
NARTKEECRDHYLKTYIE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 240 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 364 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SPCC24B10.08c protein | 1 |
Entities:
Entity 1, SPCC24B10.08c protein 58 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PHE | ASP | GLU | ||||
| 2 | ASN | TRP | GLY | ALA | ASP | GLU | GLU | LEU | LEU | LEU | ||||
| 3 | ILE | ASP | ALA | CYS | GLU | THR | LEU | GLY | LEU | GLY | ||||
| 4 | ASN | TRP | ALA | ASP | ILE | ALA | ASP | TYR | VAL | GLY | ||||
| 5 | ASN | ALA | ARG | THR | LYS | GLU | GLU | CYS | ARG | ASP | ||||
| 6 | HIS | TYR | LEU | LYS | THR | TYR | ILE | GLU |
Samples:
sample_1: SANT domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution & refinement
NMR spectrometers:
- Bruker AVANCE2 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts