BMRB Entry 10195
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10195
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Title: Solution structure of the second SH3 domain of human Vinexin
Deposition date: 2008-02-19 Original release date: 2009-02-27
Authors: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second SH3 domain of human Vinexin" . ., .-..
Assembly members:
SH3 domain, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
SH3 domain: GSSGSSGKPPTYQVLEYGEA
VAQYTFKGDLEVELSFRKGE
HICLIRKVNENWYEGRITGT
GRQGIFPASYVQVSREPRLR
LCDDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 356 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 571 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 90 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | PRO | PRO | |
| 2 | THR | TYR | GLN | VAL | LEU | GLU | TYR | GLY | GLU | ALA | |
| 3 | VAL | ALA | GLN | TYR | THR | PHE | LYS | GLY | ASP | LEU | |
| 4 | GLU | VAL | GLU | LEU | SER | PHE | ARG | LYS | GLY | GLU | |
| 5 | HIS | ILE | CYS | LEU | ILE | ARG | LYS | VAL | ASN | GLU | |
| 6 | ASN | TRP | TYR | GLU | GLY | ARG | ILE | THR | GLY | THR | |
| 7 | GLY | ARG | GLN | GLY | ILE | PHE | PRO | ALA | SER | TYR | |
| 8 | VAL | GLN | VAL | SER | ARG | GLU | PRO | ARG | LEU | ARG | |
| 9 | LEU | CYS | ASP | ASP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.00 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F - processing
NMNMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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