BMRB Entry 10252
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10252
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Title: Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse
Deposition date: 2008-11-20 Original release date: 2009-11-20
Authors: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the HMG_box domain of thymus high mobility group box protein TOX from mouse" . ., .-..
Assembly members:
HMG box, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: Cell free synthesis
Entity Sequences (FASTA):
HMG box: GSSGSSGKKKKKKDPNEPQK
PVSAYALFFRDTQAAIKGQN
PNATFGEVSKIVASMWDGLG
EEQKQVYKKKTEAAKKEYLK
QLAAYRASLVSKSYTDSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 394 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 599 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HMG box | 1 |
Entities:
Entity 1, HMG box 102 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | LYS | LYS | ||||
| 2 | LYS | LYS | LYS | ASP | PRO | ASN | GLU | PRO | GLN | LYS | ||||
| 3 | PRO | VAL | SER | ALA | TYR | ALA | LEU | PHE | PHE | ARG | ||||
| 4 | ASP | THR | GLN | ALA | ALA | ILE | LYS | GLY | GLN | ASN | ||||
| 5 | PRO | ASN | ALA | THR | PHE | GLY | GLU | VAL | SER | LYS | ||||
| 6 | ILE | VAL | ALA | SER | MET | TRP | ASP | GLY | LEU | GLY | ||||
| 7 | GLU | GLU | GLN | LYS | GLN | VAL | TYR | LYS | LYS | LYS | ||||
| 8 | THR | GLU | ALA | ALA | LYS | LYS | GLU | TYR | LEU | LYS | ||||
| 9 | GLN | LEU | ALA | ALA | TYR | ARG | ALA | SER | LEU | VAL | ||||
| 10 | SER | LYS | SER | TYR | THR | ASP | SER | GLY | PRO | SER | ||||
| 11 | SER | GLY |
Samples:
sample_1: HMG_box domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.913, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA34528 BAC26568 BAC30091 BAG11241 BAG63663 |
| EMBL | CAF93289 CBY16474 |
| GB | AAH16665 AAH80732 AAI47900 AAI66269 AAL78656 |
| REF | NP_001095566 NP_001102124 NP_001121484 NP_001248418 NP_055544 |
| SP | O94900 Q66JW3 |
| TPG | DAA22741 |
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