BMRB Entry 10258
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10258
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Title: Solution structures of the fn3 domain of human ephrin type-B receptor 1
Deposition date: 2008-11-21 Original release date: 2009-11-24
Authors: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human ephrin type-B receptor 1" . ., .-..
Assembly members:
Fibronectin type III domain, polymer, 108 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis
Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGPSTVPIMHQVSAT
MRSITLSWPQPEQPNGIILD
YEIRYYEKEHNEFNSSMARS
QTNTARIDGLRPGMVYVVQV
RARTVAGYGKFSGKMCFQTL
TDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 450 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 706 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fibronectin type III domain | 1 |
Entities:
Entity 1, Fibronectin type III domain 108 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | SER | THR | ||||
| 2 | VAL | PRO | ILE | MET | HIS | GLN | VAL | SER | ALA | THR | ||||
| 3 | MET | ARG | SER | ILE | THR | LEU | SER | TRP | PRO | GLN | ||||
| 4 | PRO | GLU | GLN | PRO | ASN | GLY | ILE | ILE | LEU | ASP | ||||
| 5 | TYR | GLU | ILE | ARG | TYR | TYR | GLU | LYS | GLU | HIS | ||||
| 6 | ASN | GLU | PHE | ASN | SER | SER | MET | ALA | ARG | SER | ||||
| 7 | GLN | THR | ASN | THR | ALA | ARG | ILE | ASP | GLY | LEU | ||||
| 8 | ARG | PRO | GLY | MET | VAL | TYR | VAL | VAL | GLN | VAL | ||||
| 9 | ARG | ALA | ARG | THR | VAL | ALA | GLY | TYR | GLY | LYS | ||||
| 10 | PHE | SER | GLY | LYS | MET | CYS | PHE | GLN | THR | LEU | ||||
| 11 | THR | ASP | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts