BMRB Entry 10266
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10266
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Title: Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F
Deposition date: 2008-12-15 Original release date: 2009-12-14
Authors: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human receptor-type tyrosine-protein phosphatase F" . ., .-..
Assembly members:
Fibronectin type III domain, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Fibronectin type III domain: GSSGSSGPSGFPQNLHVTGL
TTSTTELAWDPPVLAERNGR
IISYTVVFRDINSQQELQNI
TTDTRFTLTGLKPDTTYDIK
VRAWTSKGSGPLSPSIQSRT
MPV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 422 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 688 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fibronectin type III domain | 1 |
Entities:
Entity 1, Fibronectin type III domain 103 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | SER | GLY | ||||
| 2 | PHE | PRO | GLN | ASN | LEU | HIS | VAL | THR | GLY | LEU | ||||
| 3 | THR | THR | SER | THR | THR | GLU | LEU | ALA | TRP | ASP | ||||
| 4 | PRO | PRO | VAL | LEU | ALA | GLU | ARG | ASN | GLY | ARG | ||||
| 5 | ILE | ILE | SER | TYR | THR | VAL | VAL | PHE | ARG | ASP | ||||
| 6 | ILE | ASN | SER | GLN | GLN | GLU | LEU | GLN | ASN | ILE | ||||
| 7 | THR | THR | ASP | THR | ARG | PHE | THR | LEU | THR | GLY | ||||
| 8 | LEU | LYS | PRO | ASP | THR | THR | TYR | ASP | ILE | LYS | ||||
| 9 | VAL | ARG | ALA | TRP | THR | SER | LYS | GLY | SER | GLY | ||||
| 10 | PRO | LEU | SER | PRO | SER | ILE | GLN | SER | ARG | THR | ||||
| 11 | MET | PRO | VAL |
Samples:
sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v2.0.17, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB |
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