BMRB Entry 10283
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10283
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Title: Solution structure of the first homeobox domain of AT-binding transcription factor 1 (ATBF1)
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Ohnishi, S.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the first homeobox domain of AT-binding transcription factor 1 (ATBF1)" . ., .-..
Assembly members:
The first homeobox domain, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
The first homeobox domain: GSSGSSGKRPRTRITDDQLR
VLRQYFDINNSPSEEQIKEM
ADKSGLPQKVIKHWFRNTLF
KERQSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 294 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 434 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The first homeobox domain | 1 |
Entities:
Entity 1, The first homeobox domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | ARG | PRO | |
| 2 | ARG | THR | ARG | ILE | THR | ASP | ASP | GLN | LEU | ARG | |
| 3 | VAL | LEU | ARG | GLN | TYR | PHE | ASP | ILE | ASN | ASN | |
| 4 | SER | PRO | SER | GLU | GLU | GLN | ILE | LYS | GLU | MET | |
| 5 | ALA | ASP | LYS | SER | GLY | LEU | PRO | GLN | LYS | VAL | |
| 6 | ILE | LYS | HIS | TRP | PHE | ARG | ASN | THR | LEU | PHE | |
| 7 | LYS | GLU | ARG | GLN | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: The first homeobox domain, [U-13C; U-15N], 1.2 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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