BMRB Entry 10286
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10286
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Title: Solution structure of the homeobox domain of the hypothetical protein, DKFZp686K21156
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Ohnishi, S.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of the hypothetical protein, DKFZp686K21156" . ., .-..
Assembly members:
homeobox domain, polymer, 80 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
homeobox domain: GSSGSSGALQDRTQFSDRDL
ATLKKYWDNGMTSLGSVCRE
KIEAVATELNVDCEIVRTWI
GNRRRKYRLMGIEVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 319 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 499 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | homeobox domain | 1 |
Entities:
Entity 1, homeobox domain 80 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | LEU | GLN | |
| 2 | ASP | ARG | THR | GLN | PHE | SER | ASP | ARG | ASP | LEU | |
| 3 | ALA | THR | LEU | LYS | LYS | TYR | TRP | ASP | ASN | GLY | |
| 4 | MET | THR | SER | LEU | GLY | SER | VAL | CYS | ARG | GLU | |
| 5 | LYS | ILE | GLU | ALA | VAL | ALA | THR | GLU | LEU | ASN | |
| 6 | VAL | ASP | CYS | GLU | ILE | VAL | ARG | THR | TRP | ILE | |
| 7 | GLY | ASN | ARG | ARG | ARG | LYS | TYR | ARG | LEU | MET | |
| 8 | GLY | ILE | GLU | VAL | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts