BMRB Entry 10288
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10288
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Title: Solution structure of the homeobox domain of Hepatocyte nuclear factor 1-beta (HNF-1beta)
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Ohnishi, S.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the homeobox domain of Hepatocyte nuclear factor 1-beta (HNF-1beta)" . ., .-..
Assembly members:
homeobox domain, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
homeobox domain: GSSGSSGRNRFKWGPASQQI
LYQAYDRQKNPSKEEREALV
EECNRAECLQRGVSPSKAHG
LGSNLVTEVRVYNWFANRRK
EEAFRQKLAMDAYSSNSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 401 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 623 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | homeobox domain | 1 |
Entities:
Entity 1, homeobox domain 102 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | ASN | ARG | ||||
| 2 | PHE | LYS | TRP | GLY | PRO | ALA | SER | GLN | GLN | ILE | ||||
| 3 | LEU | TYR | GLN | ALA | TYR | ASP | ARG | GLN | LYS | ASN | ||||
| 4 | PRO | SER | LYS | GLU | GLU | ARG | GLU | ALA | LEU | VAL | ||||
| 5 | GLU | GLU | CYS | ASN | ARG | ALA | GLU | CYS | LEU | GLN | ||||
| 6 | ARG | GLY | VAL | SER | PRO | SER | LYS | ALA | HIS | GLY | ||||
| 7 | LEU | GLY | SER | ASN | LEU | VAL | THR | GLU | VAL | ARG | ||||
| 8 | VAL | TYR | ASN | TRP | PHE | ALA | ASN | ARG | ARG | LYS | ||||
| 9 | GLU | GLU | ALA | PHE | ARG | GLN | LYS | LEU | ALA | MET | ||||
| 10 | ASP | ALA | TYR | SER | SER | ASN | SER | GLY | PRO | SER | ||||
| 11 | SER | GLY |
Samples:
sample_1: homeobox domain, [U-13C; U-15N], 1.2 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA77718 BAA77719 BAA77720 BAA77721 BAB23604 |
| EMBL | CAA39358 CAA39886 CAA41652 CAA49356 CAB59223 |
| GB | AAC63388 AAH17714 AAH25189 AAH81826 AAI29148 |
| REF | NP_000449 NP_001124797 NP_001159395 NP_001179784 NP_001181477 |
| SP | A1L1N5 P23899 P27889 P35680 Q03365 |
| TPG | DAA19061 DAA19062 |
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