BMRB Entry 10296
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10296
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Title: Solution structure of the homeobox domain of Homeobox protein goosecoid
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Ohnishi, S.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain of Homeobox protein goosecoid" . ., .-..
Assembly members:
homeobox domain, polymer, 70 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
homeobox domain: GSSGSSGRRHRTIFTDEQLE
ALENLFQETKYPDVGTREQL
ARKVHLREEKVEVWFKNRRA
KWRRSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 280 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 423 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | homeobox domain | 1 |
Entities:
Entity 1, homeobox domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | ARG | HIS | |
| 2 | ARG | THR | ILE | PHE | THR | ASP | GLU | GLN | LEU | GLU | |
| 3 | ALA | LEU | GLU | ASN | LEU | PHE | GLN | GLU | THR | LYS | |
| 4 | TYR | PRO | ASP | VAL | GLY | THR | ARG | GLU | GLN | LEU | |
| 5 | ALA | ARG | LYS | VAL | HIS | LEU | ARG | GLU | GLU | LYS | |
| 6 | VAL | GLU | VAL | TRP | PHE | LYS | ASN | ARG | ARG | ALA | |
| 7 | LYS | TRP | ARG | ARG | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: homeobox domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.965, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAG73208 BAK40073 BAR79454 |
| EMBL | CAA49897 CAA73611 CAA73612 CAA76833 CAJ82436 |
| GB | AAA37826 AAA49729 AAA49744 AAA50028 AAA50030 |
| REF | NP_001016704 NP_001074955 NP_001081278 NP_001093429 NP_001107361 |
| SP | A1YEY5 A1YFI3 A1YG57 A2T733 A2T7P4 |
| TPG | DAA17416 |
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