BMRB Entry 10297
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10297
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Title: Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8
Deposition date: 2008-12-17 Original release date: 2009-12-17
Authors: Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.
Citation: Ohnishi, S.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the homeobox domain from human NIL-2-A zinc finger protein, transcription factor 8" . ., .-..
Assembly members:
homeobox domain, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
homeobox domain: GSSGSSGQPPLKNLLSLLKA
YYALNAQPSAEELSKIADSV
NLPLDVVKKWFEKMQAGQIS
VQSS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 264 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 418 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | homeobox domain | 1 |
Entities:
Entity 1, homeobox domain 64 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | PRO | PRO | ||||
| 2 | LEU | LYS | ASN | LEU | LEU | SER | LEU | LEU | LYS | ALA | ||||
| 3 | TYR | TYR | ALA | LEU | ASN | ALA | GLN | PRO | SER | ALA | ||||
| 4 | GLU | GLU | LEU | SER | LYS | ILE | ALA | ASP | SER | VAL | ||||
| 5 | ASN | LEU | PRO | LEU | ASP | VAL | VAL | LYS | LYS | TRP | ||||
| 6 | PHE | GLU | LYS | MET | GLN | ALA | GLY | GLN | ILE | SER | ||||
| 7 | VAL | GLN | SER | SER |
Samples:
sample_1: homeobox domain, [U-13C; U-15N], 1.0 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9742, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAA03259 BAA11178 |
| GB | EMC89439 EMP31625 EOA95468 KFM04765 KFO63461 |
| REF | NP_990462 XP_005027918 XP_005027919 XP_005027920 XP_005040695 |
| SP | P36197 |
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