BMRB Entry 10314

Name: Solution structure of the PH domain of Evectin-2 from mouse
Published: 2010-03-11

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PDB ID: 2dhi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10314
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the PH domain of Evectin-2 from mouse

Deposition date: 2009-03-11 Original release date: 2010-03-11

Authors: Li, H.; Yoneyama, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Yoneyama, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of Evectin-2 from mouse" . ., .-..

Assembly members:
PH domain, polymer, 120 residues, Formula weight is not available

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: cell-free synthesis

Entity Sequences (FASTA):
PH domain: GSSGSSGFVKSGWLLRQSTI LKRWKKNWFDLWSDGHLIYY DDQTRQSIEDKVHMPVDCIN IRTGHECRDIQPPDGKPRDC LLQIVCRDGKTISLCAESTD DCLAWKFTLQDSRTSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	512
15N chemical shifts	122
1H chemical shifts	806

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 120 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	PHE	VAL	LYS
2	SER	GLY	TRP	LEU	LEU	ARG	GLN	SER	THR	ILE
3	LEU	LYS	ARG	TRP	LYS	LYS	ASN	TRP	PHE	ASP
4	LEU	TRP	SER	ASP	GLY	HIS	LEU	ILE	TYR	TYR
5	ASP	ASP	GLN	THR	ARG	GLN	SER	ILE	GLU	ASP
6	LYS	VAL	HIS	MET	PRO	VAL	ASP	CYS	ILE	ASN
7	ILE	ARG	THR	GLY	HIS	GLU	CYS	ARG	ASP	ILE
8	GLN	PRO	PRO	ASP	GLY	LYS	PRO	ARG	ASP	CYS
9	LEU	LEU	GLN	ILE	VAL	CYS	ARG	ASP	GLY	LYS
10	THR	ILE	SER	LEU	CYS	ALA	GLU	SER	THR	ASP
11	ASP	CYS	LEU	ALA	TRP	LYS	PHE	THR	LEU	GLN
12	ASP	SER	ARG	THR	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DHI
DBJ	BAC38413
GB	EDL14464 EDL14465 EDL99298
REF	XP_007464518 XP_012391058

Biological Magnetic Resonance Data Bank