BMRB Entry 10317
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10317
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Title: Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human
Deposition date: 2009-03-11 Original release date: 2010-03-11
Authors: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of pleckstrin homology domain-containing protein family A member 5 from human"" . ., .-..
Assembly members:
PH domain, polymer, 128 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis
Entity Sequences (FASTA):
PH domain: GSSGSSGKRSNSIKRNPNAP
VVRRGWLYKQDSTGMKLWKK
RWFVLSDLCLFYYRDEKEEG
ILGSILLPSFQIALLTSEDH
INRKYAFKAAHPNMRTYYFC
TDTGKEMELWMKAMLDAALV
QTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 558 |
| 15N chemical shifts | 117 |
| 1H chemical shifts | 869 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pleckstrin homology domain-containing family A member 5 | 1 |
Entities:
Entity 1, Pleckstrin homology domain-containing family A member 5 128 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | ARG | SER | ||||
| 2 | ASN | SER | ILE | LYS | ARG | ASN | PRO | ASN | ALA | PRO | ||||
| 3 | VAL | VAL | ARG | ARG | GLY | TRP | LEU | TYR | LYS | GLN | ||||
| 4 | ASP | SER | THR | GLY | MET | LYS | LEU | TRP | LYS | LYS | ||||
| 5 | ARG | TRP | PHE | VAL | LEU | SER | ASP | LEU | CYS | LEU | ||||
| 6 | PHE | TYR | TYR | ARG | ASP | GLU | LYS | GLU | GLU | GLY | ||||
| 7 | ILE | LEU | GLY | SER | ILE | LEU | LEU | PRO | SER | PHE | ||||
| 8 | GLN | ILE | ALA | LEU | LEU | THR | SER | GLU | ASP | HIS | ||||
| 9 | ILE | ASN | ARG | LYS | TYR | ALA | PHE | LYS | ALA | ALA | ||||
| 10 | HIS | PRO | ASN | MET | ARG | THR | TYR | TYR | PHE | CYS | ||||
| 11 | THR | ASP | THR | GLY | LYS | GLU | MET | GLU | LEU | TRP | ||||
| 12 | MET | LYS | ALA | MET | LEU | ASP | ALA | ALA | LEU | VAL | ||||
| 13 | GLN | THR | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PH domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB21777 BAC98233 BAG10115 BAG57882 BAG58167 |
| GB | AAG22817 AAH00969 AAH70174 AAI27093 AAM44231 |
| REF | NP_001137293 NP_001243399 NP_001243716 NP_061885 NP_647556 |
| SP | Q9HAU0 |
| TPG | DAA29392 |
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