BMRB Entry 10320
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10320
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Title: Solution structure of the 9th filamin domain from human Filamin-B
Deposition date: 2009-04-02 Original release date: 2010-04-07
Authors: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 9th filamin domain from human Filamin-B" . ., .-..
Assembly members:
Filamin domain, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin domain: GSSGSSGDVTYDGHPVPGSP
YTVEASLPPDPSKVKAHGPG
LEGGLVGKPAEFTIDTKGAG
TGGLGLTVEGPCEAKIECSD
NGDGTCSVSYLPTKPGEYFV
NILFEEVHIPGSPFKADIEM
PFDPSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 520 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 782 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin domain | 1 |
Entities:
Entity 1, Filamin domain 131 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | VAL | THR | ||||
| 2 | TYR | ASP | GLY | HIS | PRO | VAL | PRO | GLY | SER | PRO | ||||
| 3 | TYR | THR | VAL | GLU | ALA | SER | LEU | PRO | PRO | ASP | ||||
| 4 | PRO | SER | LYS | VAL | LYS | ALA | HIS | GLY | PRO | GLY | ||||
| 5 | LEU | GLU | GLY | GLY | LEU | VAL | GLY | LYS | PRO | ALA | ||||
| 6 | GLU | PHE | THR | ILE | ASP | THR | LYS | GLY | ALA | GLY | ||||
| 7 | THR | GLY | GLY | LEU | GLY | LEU | THR | VAL | GLU | GLY | ||||
| 8 | PRO | CYS | GLU | ALA | LYS | ILE | GLU | CYS | SER | ASP | ||||
| 9 | ASN | GLY | ASP | GLY | THR | CYS | SER | VAL | SER | TYR | ||||
| 10 | LEU | PRO | THR | LYS | PRO | GLY | GLU | TYR | PHE | VAL | ||||
| 11 | ASN | ILE | LEU | PHE | GLU | GLU | VAL | HIS | ILE | PRO | ||||
| 12 | GLY | SER | PRO | PHE | LYS | ALA | ASP | ILE | GLU | MET | ||||
| 13 | PRO | PHE | ASP | PRO | SER | SER | GLY | PRO | SER | SER | ||||
| 14 | GLY |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9742, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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