BMRB Entry 10328
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10328
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Title: Solution structure of the 14th Filamin domain from human Filamin C
Deposition date: 2009-04-14 Original release date: 2010-04-14
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 14th Filamin domain from human Filamin C" . ., .-..
Assembly members:
Filamin domain, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin domain: GSSGSSGAHDASKVRASGPG
LNASGIPASLPVEFTIDARD
AGEGLLTVQILDPEGKPKKA
NIRDNGDGTYTVSYLPDMSG
RYTITIKYGGDEIPYSPFRI
HALPTGDASSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 467 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 720 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin domain | 1 |
Entities:
Entity 1, Filamin domain 115 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | HIS | ASP | ||||
| 2 | ALA | SER | LYS | VAL | ARG | ALA | SER | GLY | PRO | GLY | ||||
| 3 | LEU | ASN | ALA | SER | GLY | ILE | PRO | ALA | SER | LEU | ||||
| 4 | PRO | VAL | GLU | PHE | THR | ILE | ASP | ALA | ARG | ASP | ||||
| 5 | ALA | GLY | GLU | GLY | LEU | LEU | THR | VAL | GLN | ILE | ||||
| 6 | LEU | ASP | PRO | GLU | GLY | LYS | PRO | LYS | LYS | ALA | ||||
| 7 | ASN | ILE | ARG | ASP | ASN | GLY | ASP | GLY | THR | TYR | ||||
| 8 | THR | VAL | SER | TYR | LEU | PRO | ASP | MET | SER | GLY | ||||
| 9 | ARG | TYR | THR | ILE | THR | ILE | LYS | TYR | GLY | GLY | ||||
| 10 | ASP | GLU | ILE | PRO | TYR | SER | PRO | PHE | ARG | ILE | ||||
| 11 | HIS | ALA | LEU | PRO | THR | GLY | ASP | ALA | SER | SER | ||||
| 12 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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