BMRB Entry 10331
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10331
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Title: Solution structure of the 22th Filamin domain from human Filamin C
Deposition date: 2009-04-14 Original release date: 2010-04-14
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 22th Filamin domain from human Filamin C" . ., .-..
Assembly members:
Filamin domain, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin domain: GSSGSSGSDDARRLTVTSLQ
ETGLKVNQPASFAVQLNGAR
GVIDARVHTPSGAVEECYVS
ELDSDKHTIRFIPHENGVHS
IDVKFNGAHIPGSPFKIRVG
EQSQAGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 700 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin domain | 1 |
Entities:
Entity 1, Filamin domain 112 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | ASP | ASP | ||||
| 2 | ALA | ARG | ARG | LEU | THR | VAL | THR | SER | LEU | GLN | ||||
| 3 | GLU | THR | GLY | LEU | LYS | VAL | ASN | GLN | PRO | ALA | ||||
| 4 | SER | PHE | ALA | VAL | GLN | LEU | ASN | GLY | ALA | ARG | ||||
| 5 | GLY | VAL | ILE | ASP | ALA | ARG | VAL | HIS | THR | PRO | ||||
| 6 | SER | GLY | ALA | VAL | GLU | GLU | CYS | TYR | VAL | SER | ||||
| 7 | GLU | LEU | ASP | SER | ASP | LYS | HIS | THR | ILE | ARG | ||||
| 8 | PHE | ILE | PRO | HIS | GLU | ASN | GLY | VAL | HIS | SER | ||||
| 9 | ILE | ASP | VAL | LYS | PHE | ASN | GLY | ALA | HIS | ILE | ||||
| 10 | PRO | GLY | SER | PRO | PHE | LYS | ILE | ARG | VAL | GLY | ||||
| 11 | GLU | GLN | SER | GLN | ALA | GLY | SER | GLY | PRO | SER | ||||
| 12 | SER | GLY |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
or all simulated shifts