BMRB Entry 10332
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10332
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Title: Solution structure of the 23th Filamin domain from human Filamin C
Deposition date: 2009-04-14 Original release date: 2010-04-14
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 23th Filamin domain from human Filamin C" . ., .-..
Assembly members:
Filamin domain, polymer, 111 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin domain: GSSGSSGAGDPGLVSAYGPG
LEGGTTGVSSEFIVNTLNAG
SGALSVTIDGPSKVQLDCRE
CPEGHVVTYTPMAPGNYLIA
IKYGGPQHIVGSPFKAKVTG
PRLSGSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 425 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 657 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin domain | 1 |
Entities:
Entity 1, Filamin domain 111 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | GLY | ASP | ||||
| 2 | PRO | GLY | LEU | VAL | SER | ALA | TYR | GLY | PRO | GLY | ||||
| 3 | LEU | GLU | GLY | GLY | THR | THR | GLY | VAL | SER | SER | ||||
| 4 | GLU | PHE | ILE | VAL | ASN | THR | LEU | ASN | ALA | GLY | ||||
| 5 | SER | GLY | ALA | LEU | SER | VAL | THR | ILE | ASP | GLY | ||||
| 6 | PRO | SER | LYS | VAL | GLN | LEU | ASP | CYS | ARG | GLU | ||||
| 7 | CYS | PRO | GLU | GLY | HIS | VAL | VAL | THR | TYR | THR | ||||
| 8 | PRO | MET | ALA | PRO | GLY | ASN | TYR | LEU | ILE | ALA | ||||
| 9 | ILE | LYS | TYR | GLY | GLY | PRO | GLN | HIS | ILE | VAL | ||||
| 10 | GLY | SER | PRO | PHE | LYS | ALA | LYS | VAL | THR | GLY | ||||
| 11 | PRO | ARG | LEU | SER | GLY | SER | GLY | PRO | SER | SER | ||||
| 12 | GLY |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.932, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts