BMRB Entry 10333
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10333
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Title: Solution structure of the filamin domain from human BK158_1 protein
Deposition date: 2009-04-14 Original release date: 2010-04-14
Authors: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the filamin domain from human BK158_1 protein" . ., .-..
Assembly members:
Filamin-type immunoglobulin domains, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis
Entity Sequences (FASTA):
Filamin-type immunoglobulin domains: GSSGSSGETGGERQLSPEKS
EIWGPGLKADVVLPARYFYI
QAVDTSGNKFTSSPGEKVFQ
VKVSAPEEQFTRVGVQVLDR
KDGSFIVRYRMYASYKNLKV
EIKFQGQHVAKSPYILKGSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 529 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 827 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Filamin-type immunoglobulin domains | 1 |
Entities:
Entity 1, Filamin-type immunoglobulin domains 124 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | THR | GLY | ||||
| 2 | GLY | GLU | ARG | GLN | LEU | SER | PRO | GLU | LYS | SER | ||||
| 3 | GLU | ILE | TRP | GLY | PRO | GLY | LEU | LYS | ALA | ASP | ||||
| 4 | VAL | VAL | LEU | PRO | ALA | ARG | TYR | PHE | TYR | ILE | ||||
| 5 | GLN | ALA | VAL | ASP | THR | SER | GLY | ASN | LYS | PHE | ||||
| 6 | THR | SER | SER | PRO | GLY | GLU | LYS | VAL | PHE | GLN | ||||
| 7 | VAL | LYS | VAL | SER | ALA | PRO | GLU | GLU | GLN | PHE | ||||
| 8 | THR | ARG | VAL | GLY | VAL | GLN | VAL | LEU | ASP | ARG | ||||
| 9 | LYS | ASP | GLY | SER | PHE | ILE | VAL | ARG | TYR | ARG | ||||
| 10 | MET | TYR | ALA | SER | TYR | LYS | ASN | LEU | LYS | VAL | ||||
| 11 | GLU | ILE | LYS | PHE | GLN | GLY | GLN | HIS | VAL | ALA | ||||
| 12 | LYS | SER | PRO | TYR | ILE | LEU | LYS | GLY | SER | GLY | ||||
| 13 | PRO | SER | SER | GLY |
Samples:
sample_1: Filamin domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9742, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96287 |
| GB | AAH01297 AAQ89318 ABM82133 ABM85316 AIC57069 |
| REF | NP_076994 XP_001094880 XP_002824457 XP_003832113 XP_003919616 |
| SP | Q6UW63 |
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