BMRB Entry 11044
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR11044
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Title: Solution structure of Magi4, a spider toxin from Macrothele gigas PubMed: 19592486
Deposition date: 2008-04-13 Original release date: 2009-07-17
Authors: Yamaji, Nahoko
Citation: Yamaji, Nahoko; Little, Michelle; Nishio, Hideki; Billen, Bert; Villegas, Elba; Nishiuchi, Yuji; Tytgat, Jan; Nicholson, Graham; Corzo, Gerardo. "Synthesis, Solution Structure, and Phylum Selectivity of a Spider {delta}-Toxin That Slows Inactivation of Specific Voltage-gated Sodium Channel Subtypes" J. Biol. Chem. 284, 24568-24582 (2009).
Assembly members:
magi4, polymer, 43 residues, Formula weight is not available
Natural source: Common Name: Macrothele gigas Taxonomy ID: 223896 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macrothele gigas
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
magi4: CGSKRAWCKEKKDCCCGYNC
VYAWYNQQSSCERKWKYLFT
GEC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 286 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | magi4 | 1 |
Entities:
Entity 1, magi4 43 residues - Formula weight is not available
| 1 | CYS | GLY | SER | LYS | ARG | ALA | TRP | CYS | LYS | GLU | ||||
| 2 | LYS | LYS | ASP | CYS | CYS | CYS | GLY | TYR | ASN | CYS | ||||
| 3 | VAL | TYR | ALA | TRP | TYR | ASN | GLN | GLN | SER | SER | ||||
| 4 | CYS | GLU | ARG | LYS | TRP | LYS | TYR | LEU | PHE | THR | ||||
| 5 | GLY | GLU | CYS |
Samples:
sample_1: magi4 3.7 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 2.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.9.1, Schwieters, Kuszewski, Tjandra and Clore - structure solution
ANSIG v3.3, Kraulis - chemical shift assignment, peak picking
xwinnmr v2.5, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker DMX 750 MHz
- Bruker DMX 500 MHz