BMRB Entry 11092
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11092
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Title: Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human
Deposition date: 2010-01-18 Original release date: 2011-01-19
Authors: Li, H.; Sato, M.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.
Citation: Li, H.; Sato, M.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human" . ., .-..
Assembly members:
Chromo domain, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Chromo domain: GSSGSSGMAPKQDPKPKFQE
GERVLCFHGPLLYEAKCVKV
AIKDKQVKYFIHYSGWNKNW
DEWVPESRVLKYVDTNLQKQ
RELQKANQEQYAEGKMRGAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 439 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 692 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Chromo domain | 1 |
Entities:
Entity 1, Chromo domain 100 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | ALA | PRO | |
| 2 | LYS | GLN | ASP | PRO | LYS | PRO | LYS | PHE | GLN | GLU | |
| 3 | GLY | GLU | ARG | VAL | LEU | CYS | PHE | HIS | GLY | PRO | |
| 4 | LEU | LEU | TYR | GLU | ALA | LYS | CYS | VAL | LYS | VAL | |
| 5 | ALA | ILE | LYS | ASP | LYS | GLN | VAL | LYS | TYR | PHE | |
| 6 | ILE | HIS | TYR | SER | GLY | TRP | ASN | LYS | ASN | TRP | |
| 7 | ASP | GLU | TRP | VAL | PRO | GLU | SER | ARG | VAL | LEU | |
| 8 | LYS | TYR | VAL | ASP | THR | ASN | LEU | GLN | LYS | GLN | |
| 9 | ARG | GLU | LEU | GLN | LYS | ALA | ASN | GLN | GLU | GLN | |
| 10 | TYR | ALA | GLU | GLY | LYS | MET | ARG | GLY | ALA | ALA |
Samples:
sample_1: Chromo domain, [U-13C; U-15N], 1.13 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9827, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB30219 BAB58904 BAE00783 BAE26980 BAE27430 |
| EMBL | CAB70879 CAI29614 |
| GB | AAD20970 AAD29872 AAF29033 AAF80854 AAG17253 |
| REF | NP_001011999 NP_001030525 NP_001032250 NP_001032253 NP_001127679 |
| SP | Q5NVP9 Q6AYU1 |
| TPG | DAA17605 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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