BMRB Entry 11130

Name: Solution structure of two zf-C2H2 domains from human Zinc finger protein 512
Published: 2011-04-01

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PDB ID: 2ctd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11130
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of two zf-C2H2 domains from human Zinc finger protein 512

Deposition date: 2010-03-31 Original release date: 2011-04-01

Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of two zf-C2H2 domains from human Zinc finger protein 512" . ., .-..

Assembly members:
zf-C2H2 domains, polymer, 96 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free

Entity Sequences (FASTA):
zf-C2H2 domains: GSSGSSGRIRKEPPVYAAGS LEEQWYLEIVDKGSVSCPTC QAVGRKTIEGLKKHMENCKQ EMFTCHHCGKQLRSLAGMKY HVMANHNSLPSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	386
15N chemical shifts	89
1H chemical shifts	610

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zf-C2H2 domains	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, zf-C2H2 domains 96 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

ILE

ARG

2

LYS

GLU

PRO

VAL

TYR

ALA

GLY

SER

3

LEU

GLU

GLN

TRP

TYR

LEU

GLU

ILE

VAL

4

ASP

LYS

GLY

SER

VAL

SER

CYS

PRO

THR

CYS

5

GLN

ALA

VAL

GLY

ARG

LYS

THR

ILE

GLU

GLY

6

LEU

LYS

HIS

MET

GLU

ASN

CYS

LYS

GLN

7

GLU

MET

PHE

THR

CYS

HIS

CYS

GLY

LYS

8

GLN

LEU

ARG

SER

LEU

ALA

GLY

MET

LYS

TYR

9

HIS

VAL

MET

ALA

ASN

HIS

ASN

SER

LEU

PRO

10

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-C2H2 domains, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.25 mM; IDA 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CTD
GB	EHH21997 EHH55467
REF	XP_007191412 XP_008831438 XP_010989556 XP_014418967

Biological Magnetic Resonance Data Bank