BMRB Entry 11131
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11131
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Title: Solution structure of the FYVE domain from human FYVE domain containing 27 isoform b protein
Deposition date: 2010-03-31 Original release date: 2011-04-01
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the FYVE domain from human FYVE domain containing 27 isoform b protein" . ., .-..
Assembly members:
FYVE domain, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
FYVE domain: GSSGSSGRYPTNNFGNCTGC
SATFSVLKKRRSCSNCGNSF
CSRCCSFKVPKSSMGATAPE
AQRETVFVCASCNQTLSKSG
PSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 311 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 467 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FYVE domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, FYVE domain 84 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | TYR | PRO | ||||
| 2 | THR | ASN | ASN | PHE | GLY | ASN | CYS | THR | GLY | CYS | ||||
| 3 | SER | ALA | THR | PHE | SER | VAL | LEU | LYS | LYS | ARG | ||||
| 4 | ARG | SER | CYS | SER | ASN | CYS | GLY | ASN | SER | PHE | ||||
| 5 | CYS | SER | ARG | CYS | CYS | SER | PHE | LYS | VAL | PRO | ||||
| 6 | LYS | SER | SER | MET | GLY | ALA | THR | ALA | PRO | GLU | ||||
| 7 | ALA | GLN | ARG | GLU | THR | VAL | PHE | VAL | CYS | ALA | ||||
| 8 | SER | CYS | ASN | GLN | THR | LEU | SER | LYS | SER | GLY | ||||
| 9 | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-AN1 domain, [U-13C; U-15N], 1.83 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.25 mM; IDA 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC05200 BAC11260 BAH12306 BAH12390 BAH13112 |
| EMBL | CAD38913 CAH92258 |
| GB | AAH30621 AAH61741 AIC61199 AKI69883 EAW49895 |
| REF | NP_001002261 NP_001002262 NP_001126322 NP_001167590 NP_001167591 |
| SP | Q5R7K2 Q5T4F4 Q6P7B7 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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