BMRB Entry 11132
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11132
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Title: Solution structure of the HMG box like domain from human hypothetical protein FLJ14904
Deposition date: 2010-03-31 Original release date: 2011-04-01
Authors: Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.. "Solution structure of the HMG box like domain from human hypothetical protein FLJ14904" . ., .-..
Assembly members:
HMG box like domain, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
HMG box like domain: GSSGSSGMPNRKASRNAYYF
FVQEKIPELRRRGLPVARVA
DAIPYCSSDWALLREEEKEK
YAEMAREWRAAQGKDPGPSE
KQKPVFTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 383 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 592 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HMG box like domain | 1 |
Entities:
Entity 1, HMG box like domain 93 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | PRO | ASN | ||||
| 2 | ARG | LYS | ALA | SER | ARG | ASN | ALA | TYR | TYR | PHE | ||||
| 3 | PHE | VAL | GLN | GLU | LYS | ILE | PRO | GLU | LEU | ARG | ||||
| 4 | ARG | ARG | GLY | LEU | PRO | VAL | ALA | ARG | VAL | ALA | ||||
| 5 | ASP | ALA | ILE | PRO | TYR | CYS | SER | SER | ASP | TRP | ||||
| 6 | ALA | LEU | LEU | ARG | GLU | GLU | GLU | LYS | GLU | LYS | ||||
| 7 | TYR | ALA | GLU | MET | ALA | ARG | GLU | TRP | ARG | ALA | ||||
| 8 | ALA | GLN | GLY | LYS | ASP | PRO | GLY | PRO | SER | GLU | ||||
| 9 | LYS | GLN | LYS | PRO | VAL | PHE | THR | SER | GLY | PRO | ||||
| 10 | SER | SER | GLY |
Samples:
sample_1: HMG box like domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB55385 BAE01036 BAJ20777 |
| GB | AAH28595 AAY82463 ADQ32386 AIC52639 EAW90780 |
| REF | NP_001247890 NP_116247 XP_002809887 XP_003785786 XP_003893548 |
| SP | Q4R786 Q96JY0 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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