BMRB Entry 11137
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11137
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Title: Solution structure of the 14th KH type I domain from human Vigilin
Deposition date: 2010-03-31 Original release date: 2011-04-01
Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 14th KH type I domain from human Vigilin" . ., .-..
Assembly members:
KH domain, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
KH domain: GSSGSSGRIVGELEQMVSED
VPLDHRVHARIIGARGKAIR
KIMDEFKVDIRFPQSGAPDP
NCVTVTGLPENVEEAIDHIL
NLEEEYLADSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 391 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 625 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KH domain | 1 |
Entities:
Entity 1, KH domain 95 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | ILE | VAL | ||||
| 2 | GLY | GLU | LEU | GLU | GLN | MET | VAL | SER | GLU | ASP | ||||
| 3 | VAL | PRO | LEU | ASP | HIS | ARG | VAL | HIS | ALA | ARG | ||||
| 4 | ILE | ILE | GLY | ALA | ARG | GLY | LYS | ALA | ILE | ARG | ||||
| 5 | LYS | ILE | MET | ASP | GLU | PHE | LYS | VAL | ASP | ILE | ||||
| 6 | ARG | PHE | PRO | GLN | SER | GLY | ALA | PRO | ASP | PRO | ||||
| 7 | ASN | CYS | VAL | THR | VAL | THR | GLY | LEU | PRO | GLU | ||||
| 8 | ASN | VAL | GLU | GLU | ALA | ILE | ASP | HIS | ILE | LEU | ||||
| 9 | ASN | LEU | GLU | GLU | GLU | TYR | LEU | ALA | ASP | SER | ||||
| 10 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: KH domain, [U-13C; U-15N], 1.33 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
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