BMRB Entry 11180
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11180
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Title: Solution structure of the first SH3 domain of KIAA0318 protein
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first SH3 domain of KIAA0318 protein" . ., .-..
Assembly members:
SH3 domain, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGTSKQRYSGKVHLC
VARYSYNPFDGPNENPEAEL
PLTAGKYLYVYGDMDEDGFY
EGELLDGQRGLVPSNFVDFV
QDNESRLASTSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 404 |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 611 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 96 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | THR | SER | LYS | ||||
| 2 | GLN | ARG | TYR | SER | GLY | LYS | VAL | HIS | LEU | CYS | ||||
| 3 | VAL | ALA | ARG | TYR | SER | TYR | ASN | PRO | PHE | ASP | ||||
| 4 | GLY | PRO | ASN | GLU | ASN | PRO | GLU | ALA | GLU | LEU | ||||
| 5 | PRO | LEU | THR | ALA | GLY | LYS | TYR | LEU | TYR | VAL | ||||
| 6 | TYR | GLY | ASP | MET | ASP | GLU | ASP | GLY | PHE | TYR | ||||
| 7 | GLU | GLY | GLU | LEU | LEU | ASP | GLY | GLN | ARG | GLY | ||||
| 8 | LEU | VAL | PRO | SER | ASN | PHE | VAL | ASP | PHE | VAL | ||||
| 9 | GLN | ASP | ASN | GLU | SER | ARG | LEU | ALA | SER | THR | ||||
| 10 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.26 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8992, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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