BMRB Entry 11192
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11192
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the Ig-like domain(433- 525) of murine myosin-binding protein C, fast-type
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Izumi, K.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the Ig-like domain(433 - 525) of murine myosin-binding protein C, fast-type" . ., .-..
Assembly members:
Ig-like domain, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ig-like domain: GSSGSSGQLEVLQDIADLTV
KAAEQAVFKCEVSDEKVTGK
WYKNGVEVRPSKRITISHVG
RFHKLVIDDVRPEDEGDYTF
VPDGYALSLSAKLNFLEIKV
SGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 460 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 717 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ig-like domain | 1 |
Entities:
Entity 1, Ig-like domain 106 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | LEU | GLU | ||||
| 2 | VAL | LEU | GLN | ASP | ILE | ALA | ASP | LEU | THR | VAL | ||||
| 3 | LYS | ALA | ALA | GLU | GLN | ALA | VAL | PHE | LYS | CYS | ||||
| 4 | GLU | VAL | SER | ASP | GLU | LYS | VAL | THR | GLY | LYS | ||||
| 5 | TRP | TYR | LYS | ASN | GLY | VAL | GLU | VAL | ARG | PRO | ||||
| 6 | SER | LYS | ARG | ILE | THR | ILE | SER | HIS | VAL | GLY | ||||
| 7 | ARG | PHE | HIS | LYS | LEU | VAL | ILE | ASP | ASP | VAL | ||||
| 8 | ARG | PRO | GLU | ASP | GLU | GLY | ASP | TYR | THR | PHE | ||||
| 9 | VAL | PRO | ASP | GLY | TYR | ALA | LEU | SER | LEU | SER | ||||
| 10 | ALA | LYS | LEU | ASN | PHE | LEU | GLU | ILE | LYS | VAL | ||||
| 11 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: Ig-like domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta NMR v4.3.2, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.93191, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts