BMRB Entry 11194
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11194
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Title: Solution structure of the fifth SH3 domain from human KIAA0418 protein
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fifth SH3 domain from human KIAA0418 protein" . ., .-..
Assembly members:
SH3 domain, polymer, 75 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
SH3 domain: GSSGSSGNLKDVYVSIADYE
GDEETAGFQEGVSMEVLERN
PNGWWYCQILDGVKPFKGWV
PSNYLEKKNSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 294 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 455 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 75 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | LEU | LYS | ||||
| 2 | ASP | VAL | TYR | VAL | SER | ILE | ALA | ASP | TYR | GLU | ||||
| 3 | GLY | ASP | GLU | GLU | THR | ALA | GLY | PHE | GLN | GLU | ||||
| 4 | GLY | VAL | SER | MET | GLU | VAL | LEU | GLU | ARG | ASN | ||||
| 5 | PRO | ASN | GLY | TRP | TRP | TYR | CYS | GLN | ILE | LEU | ||||
| 6 | ASP | GLY | VAL | LYS | PRO | PHE | LYS | GLY | TRP | VAL | ||||
| 7 | PRO | SER | ASN | TYR | LEU | GLU | LYS | LYS | ASN | SER | ||||
| 8 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta NMR v4.3.2, JEOL - collection
NMRPipe v20060324, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9807, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Related Database Links:
| PDB |
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