BMRB Entry 11197
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11197
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Title: Solution structure of the sixth PDZ domain of human InaD-like protein
Deposition date: 2010-07-21 Original release date: 2011-07-20
Authors: Inoue, K.; Hayashi, F.; Yokoyama, S.
Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the sixth PDZ domain of human InaD-like protein" . ., .-..
Assembly members:
PDZ domain, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGPNFSHWGPPRIVE
IFREPNVSLGISIVGGQTVI
KRLKNGEELKGIFIKQVLED
SPAGKTNALKTGDKILEVSG
VDLQNASHSEAVEAIKNAGN
PVVFIVQSLSSTPRVIP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 481 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 792 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain | 1 |
Entities:
Entity 1, PDZ domain 117 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | ASN | PHE | ||||
| 2 | SER | HIS | TRP | GLY | PRO | PRO | ARG | ILE | VAL | GLU | ||||
| 3 | ILE | PHE | ARG | GLU | PRO | ASN | VAL | SER | LEU | GLY | ||||
| 4 | ILE | SER | ILE | VAL | GLY | GLY | GLN | THR | VAL | ILE | ||||
| 5 | LYS | ARG | LEU | LYS | ASN | GLY | GLU | GLU | LEU | LYS | ||||
| 6 | GLY | ILE | PHE | ILE | LYS | GLN | VAL | LEU | GLU | ASP | ||||
| 7 | SER | PRO | ALA | GLY | LYS | THR | ASN | ALA | LEU | LYS | ||||
| 8 | THR | GLY | ASP | LYS | ILE | LEU | GLU | VAL | SER | GLY | ||||
| 9 | VAL | ASP | LEU | GLN | ASN | ALA | SER | HIS | SER | GLU | ||||
| 10 | ALA | VAL | GLU | ALA | ILE | LYS | ASN | ALA | GLY | ASN | ||||
| 11 | PRO | VAL | VAL | PHE | ILE | VAL | GLN | SER | LEU | SER | ||||
| 12 | SER | THR | PRO | ARG | VAL | ILE | PRO |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20060324, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9807, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts