BMRB Entry 11200
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11200
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Title: Solution structure of the PDZ domain of Pals1 protein
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of Pals1 protein" . ., .-..
Assembly members:
PDZ domain, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGPITDERVYESIGH
YGGETVKIVRIEKARDIPLG
ATVRNEMDSVIISRIVKGGA
AEKSGLLHEGDEVLEINGIE
IRGKDVNEVFDLLSDMHGTL
TFVLIPSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 478 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 766 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MAGUK p55 subfamily member 5 | 1 |
Entities:
Entity 1, MAGUK p55 subfamily member 5 113 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | ILE | THR | ||||
| 2 | ASP | GLU | ARG | VAL | TYR | GLU | SER | ILE | GLY | HIS | ||||
| 3 | TYR | GLY | GLY | GLU | THR | VAL | LYS | ILE | VAL | ARG | ||||
| 4 | ILE | GLU | LYS | ALA | ARG | ASP | ILE | PRO | LEU | GLY | ||||
| 5 | ALA | THR | VAL | ARG | ASN | GLU | MET | ASP | SER | VAL | ||||
| 6 | ILE | ILE | SER | ARG | ILE | VAL | LYS | GLY | GLY | ALA | ||||
| 7 | ALA | GLU | LYS | SER | GLY | LEU | LEU | HIS | GLU | GLY | ||||
| 8 | ASP | GLU | VAL | LEU | GLU | ILE | ASN | GLY | ILE | GLU | ||||
| 9 | ILE | ARG | GLY | LYS | ASP | VAL | ASN | GLU | VAL | PHE | ||||
| 10 | ASP | LEU | LEU | SER | ASP | MET | HIS | GLY | THR | LEU | ||||
| 11 | THR | PHE | VAL | LEU | ILE | PRO | SER | SER | GLY | PRO | ||||
| 12 | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.05 mM; PiNa 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20020425, Delaglio.F. - processing
NMRView v5.0.4, JohnsonB.A. - data analysis
Kujira v0.854, KobayashiN. - data analysis
CYANA v1.0.7, GuntertP. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB14172 BAC05295 BAF83929 BAG11109 |
| EMBL | CAD38620 CAD89937 CAH90105 CAL37477 CAL37737 |
| GB | AAF63789 AAH53366 AAH95485 AAI29934 AAI38625 |
| REF | NP_001101504 NP_001125010 NP_001192880 NP_001243479 NP_062525 |
| SP | Q5RDQ2 Q8N3R9 Q9JLB2 |
| TPG | DAA25125 |
Download simulated HSQC data in one of the following formats:
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