BMRB Entry 11210
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11210
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Title: Solution structure of the first PDZ domain of amyloid beta A4 precursor protein-binding family A, member 1
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first PDZ domain of amyloid beta A4 precursor protein-binding family A, member 1" . ., .-..
Assembly members:
PDZ domain, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGDVFIEKQKGEILG
VVIVESGWGSILPTVIIANM
MHGGPAEKSGKLNIGDQIMS
INGTSLVGLPLSTCQSIIKG
LKNQSRVKLNIVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 406 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 671 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain of amyloid beta (A4) precursor protein-binding, family A, member 1 (X11) | 1 |
Entities:
Entity 1, PDZ domain of amyloid beta (A4) precursor protein-binding, family A, member 1 (X11) 98 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | VAL | PHE | ||||
| 2 | ILE | GLU | LYS | GLN | LYS | GLY | GLU | ILE | LEU | GLY | ||||
| 3 | VAL | VAL | ILE | VAL | GLU | SER | GLY | TRP | GLY | SER | ||||
| 4 | ILE | LEU | PRO | THR | VAL | ILE | ILE | ALA | ASN | MET | ||||
| 5 | MET | HIS | GLY | GLY | PRO | ALA | GLU | LYS | SER | GLY | ||||
| 6 | LYS | LEU | ASN | ILE | GLY | ASP | GLN | ILE | MET | SER | ||||
| 7 | ILE | ASN | GLY | THR | SER | LEU | VAL | GLY | LEU | PRO | ||||
| 8 | LEU | SER | THR | CYS | GLN | SER | ILE | ILE | LYS | GLY | ||||
| 9 | LEU | LYS | ASN | GLN | SER | ARG | VAL | LYS | LEU | ASN | ||||
| 10 | ILE | VAL | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.55 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio F.r - processing
NMRView v5.0.4, Johnson B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE28008 BAE28031 |
| GB | AAC05303 AAC39766 AAI41182 AAI56048 AAI57031 |
| REF | NP_001154 NP_113967 NP_796008 XP_001138890 XP_001365486 |
| SP | B2RUJ5 O35430 Q02410 |
Download simulated HSQC data in one of the following formats:
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