BMRB Entry 11216
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11216
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Title: Solution structure of the third PDZ domain of PDZ domain containing protein 1
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the third PDZ domain of PDZ domain containing protein 1" . ., .-..
Assembly members:
PDZ domain, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGRVVVIKKGSNGYG
FYLRAGPEQKGQIIKDIEPG
SPAEAAGLKNNDLVVAVNGK
SVEALDHDGVVEMIRKGGDQ
TTLLVLDKEAESIYSLSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 410 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 663 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain containing protein 1 | 1 |
Entities:
Entity 1, PDZ domain containing protein 1 102 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | VAL | VAL | ||||
| 2 | VAL | ILE | LYS | LYS | GLY | SER | ASN | GLY | TYR | GLY | ||||
| 3 | PHE | TYR | LEU | ARG | ALA | GLY | PRO | GLU | GLN | LYS | ||||
| 4 | GLY | GLN | ILE | ILE | LYS | ASP | ILE | GLU | PRO | GLY | ||||
| 5 | SER | PRO | ALA | GLU | ALA | ALA | GLY | LEU | LYS | ASN | ||||
| 6 | ASN | ASP | LEU | VAL | VAL | ALA | VAL | ASN | GLY | LYS | ||||
| 7 | SER | VAL | GLU | ALA | LEU | ASP | HIS | ASP | GLY | VAL | ||||
| 8 | VAL | GLU | MET | ILE | ARG | LYS | GLY | GLY | ASP | GLN | ||||
| 9 | THR | THR | LEU | LEU | VAL | LEU | ASP | LYS | GLU | ALA | ||||
| 10 | GLU | SER | ILE | TYR | SER | LEU | SER | GLY | PRO | SER | ||||
| 11 | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 900 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts