BMRB Entry 11223
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11223
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the Ig-like domain of human Leucine-rich repeat-containing protein 4
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the Ig-like domain of human Leucine-rich repeat-containing protein 4" . ., .-..
Assembly members:
IG-like domain, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
IG-like domain: GSSGSSGPFIMDAPRDLNIS
EGRMAELKCRTPPMSSVKWL
LPNGTVLSHASRHPRISVLN
DGTLNFSHVLLSDTGVYTCM
VTNVAGNSNASAYLNVSSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 414 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 654 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IG-like domain | 1 |
Entities:
Entity 1, IG-like domain 103 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | PHE | ILE | ||||
| 2 | MET | ASP | ALA | PRO | ARG | ASP | LEU | ASN | ILE | SER | ||||
| 3 | GLU | GLY | ARG | MET | ALA | GLU | LEU | LYS | CYS | ARG | ||||
| 4 | THR | PRO | PRO | MET | SER | SER | VAL | LYS | TRP | LEU | ||||
| 5 | LEU | PRO | ASN | GLY | THR | VAL | LEU | SER | HIS | ALA | ||||
| 6 | SER | ARG | HIS | PRO | ARG | ILE | SER | VAL | LEU | ASN | ||||
| 7 | ASP | GLY | THR | LEU | ASN | PHE | SER | HIS | VAL | LEU | ||||
| 8 | LEU | SER | ASP | THR | GLY | VAL | TYR | THR | CYS | MET | ||||
| 9 | VAL | THR | ASN | VAL | ALA | GLY | ASN | SER | ASN | ALA | ||||
| 10 | SER | ALA | TYR | LEU | ASN | VAL | SER | SER | GLY | PRO | ||||
| 11 | SER | SER | GLY |
Samples:
sample_1: IG-like domain, [U-13C; U-15N], 1.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts