BMRB Entry 11226
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11226
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Title: Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2" . ., .-..
Assembly members:
PDZ domain, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PDZ domain: GSSGSSGRMVGIRKTAGEHL
GVTFRVEGGELVIARILHGG
MVAQQGLLHVGDIIKEVNGQ
PVGSDPRALQELLRNASGSV
ILKILSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 365 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ domain | 1 |
Entities:
Entity 1, PDZ domain 91 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | MET | VAL | ||||
| 2 | GLY | ILE | ARG | LYS | THR | ALA | GLY | GLU | HIS | LEU | ||||
| 3 | GLY | VAL | THR | PHE | ARG | VAL | GLU | GLY | GLY | GLU | ||||
| 4 | LEU | VAL | ILE | ALA | ARG | ILE | LEU | HIS | GLY | GLY | ||||
| 5 | MET | VAL | ALA | GLN | GLN | GLY | LEU | LEU | HIS | VAL | ||||
| 6 | GLY | ASP | ILE | ILE | LYS | GLU | VAL | ASN | GLY | GLN | ||||
| 7 | PRO | VAL | GLY | SER | ASP | PRO | ARG | ALA | LEU | GLN | ||||
| 8 | GLU | LEU | LEU | ARG | ASN | ALA | SER | GLY | SER | VAL | ||||
| 9 | ILE | LEU | LYS | ILE | LEU | SER | GLY | PRO | SER | SER | ||||
| 10 | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
Delta v4.3, JEOL - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9816, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- JEOL ECA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD97280 BAE24680 BAE36473 BAE42909 BAG57760 |
| EMBL | CAA58067 CAB66489 CAI56746 |
| GB | AAC78484 AAD44342 AAH30287 AAH53026 AAX29754 |
| REF | NP_001180000 NP_001265299 NP_001265300 NP_001265301 NP_001265302 |
| SP | Q14168 Q9WV34 |
| TPG | DAA18383 |
Download simulated HSQC data in one of the following formats:
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