BMRB Entry 11230
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11230
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Title: Solution structure of the 6th Ig-like domain from human KIAA1556
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the 6th Ig-like domain from human KIAA1556" . ., .-..
Assembly members:
Ig-like domain, residues 8-97, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
Ig-like domain, residues 8-97: GSSGSSGPVRFQEALKDLEV
LEGGAATLRCVLSSVAAPVK
WCYGNNVLRPGDKYSLRQEG
AMLELVVRNLRPQDSGRYSC
SFGDQTTSATLTVTALPSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 407 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 647 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ig-like domain, residues 8-97 | 1 |
Entities:
Entity 1, Ig-like domain, residues 8-97 103 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | VAL | ARG | ||||
| 2 | PHE | GLN | GLU | ALA | LEU | LYS | ASP | LEU | GLU | VAL | ||||
| 3 | LEU | GLU | GLY | GLY | ALA | ALA | THR | LEU | ARG | CYS | ||||
| 4 | VAL | LEU | SER | SER | VAL | ALA | ALA | PRO | VAL | LYS | ||||
| 5 | TRP | CYS | TYR | GLY | ASN | ASN | VAL | LEU | ARG | PRO | ||||
| 6 | GLY | ASP | LYS | TYR | SER | LEU | ARG | GLN | GLU | GLY | ||||
| 7 | ALA | MET | LEU | GLU | LEU | VAL | VAL | ARG | ASN | LEU | ||||
| 8 | ARG | PRO | GLN | ASP | SER | GLY | ARG | TYR | SER | CYS | ||||
| 9 | SER | PHE | GLY | ASP | GLN | THR | THR | SER | ALA | THR | ||||
| 10 | LEU | THR | VAL | THR | ALA | LEU | PRO | SER | GLY | PRO | ||||
| 11 | SER | SER | GLY |
Samples:
sample_1: Ig-like domain, residues 8-97, [U-13C; U-15N], 0.39 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9816, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 900 MHz
Related Database Links:
| PDB |
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