BMRB Entry 11232
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11232
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Title: Solution structure of the PKD domain (329-428) from human KIAA0319
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PKD domain (329-428) from human KIAA0319" . ., .-..
Assembly members:
PKD domain, polymer, 113 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
PKD domain: GSSGSSGPRTVKELTVSAGD
NLIITLPDNEVELKAFVAPA
PPVETTYNYEWNLISHPTDY
QGEIKQGHKQTLNLSQLSVG
LYVFKVTVSSENAFGEGFVN
VTVKPARSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 475 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 750 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PKD domain | 1 |
Entities:
Entity 1, PKD domain 113 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | ARG | THR | ||||
| 2 | VAL | LYS | GLU | LEU | THR | VAL | SER | ALA | GLY | ASP | ||||
| 3 | ASN | LEU | ILE | ILE | THR | LEU | PRO | ASP | ASN | GLU | ||||
| 4 | VAL | GLU | LEU | LYS | ALA | PHE | VAL | ALA | PRO | ALA | ||||
| 5 | PRO | PRO | VAL | GLU | THR | THR | TYR | ASN | TYR | GLU | ||||
| 6 | TRP | ASN | LEU | ILE | SER | HIS | PRO | THR | ASP | TYR | ||||
| 7 | GLN | GLY | GLU | ILE | LYS | GLN | GLY | HIS | LYS | GLN | ||||
| 8 | THR | LEU | ASN | LEU | SER | GLN | LEU | SER | VAL | GLY | ||||
| 9 | LEU | TYR | VAL | PHE | LYS | VAL | THR | VAL | SER | SER | ||||
| 10 | GLU | ASN | ALA | PHE | GLY | GLU | GLY | PHE | VAL | ASN | ||||
| 11 | VAL | THR | VAL | LYS | PRO | ALA | ARG | SER | GLY | PRO | ||||
| 12 | SER | SER | GLY |
Samples:
sample_1: PKD domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9816, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts