BMRB Entry 11246

Name: Solution structure of the FHA domain of mouse Afadin 6
Published: 2011-08-03

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PDB ID: 1wln
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11246
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of the FHA domain of mouse Afadin 6

Deposition date: 2010-07-23 Original release date: 2011-08-03

Authors: Hayashi, F.; Hirata, Y.; Guntert, P.; Yokoyama, S.

Citation: Hayashi, F.; Hirata, Y.; Guntert, P.; Yokoyama, S.. "Solution structure of the FHA domain of mouse Afadin 6" . ., .-..

Assembly members:
FHA domain, polymer, 120 residues, Formula weight is not available

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free

Entity Sequences (FASTA):
FHA domain: GSSGSSGPEKLPYLVELSPD GSDSRDKPKLYRLQLSVTEV GTEKFDDNSIQLFGPGIQPH HCDLTNMDGVVTVTPRSMDA ETYVDGQRISETTMLQSGMR LQFGTSHVFKFVDPSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	518
15N chemical shifts	122
1H chemical shifts	805

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FHA domain	1

Entities:

Entity 1, FHA domain 120 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	PRO	GLU	LYS
2	LEU	PRO	TYR	LEU	VAL	GLU	LEU	SER	PRO	ASP
3	GLY	SER	ASP	SER	ARG	ASP	LYS	PRO	LYS	LEU
4	TYR	ARG	LEU	GLN	LEU	SER	VAL	THR	GLU	VAL
5	GLY	THR	GLU	LYS	PHE	ASP	ASP	ASN	SER	ILE
6	GLN	LEU	PHE	GLY	PRO	GLY	ILE	GLN	PRO	HIS
7	HIS	CYS	ASP	LEU	THR	ASN	MET	ASP	GLY	VAL
8	VAL	THR	VAL	THR	PRO	ARG	SER	MET	ASP	ALA
9	GLU	THR	TYR	VAL	ASP	GLY	GLN	ARG	ILE	SER
10	GLU	THR	THR	MET	LEU	GLN	SER	GLY	MET	ARG
11	LEU	GLN	PHE	GLY	THR	SER	HIS	VAL	PHE	LYS
12	PHE	VAL	ASP	PRO	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: FHA domain, [U-13C; U-15N], 0.7 mM; phosphate 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.902, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1WLN
GB	EGW13515 EHA98723 ERE82130
REF	XP_006523823 XP_006523825 XP_006523830 XP_006975980 XP_007607738

Biological Magnetic Resonance Data Bank