BMRB Entry 11260
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11260
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Title: Solution Structure of UBA domain of Human Ubiquitin Associated Protein 1 (UBAP1)
Deposition date: 2010-08-09 Original release date: 2011-08-17
Authors: Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of UBA domain of Human Ubiquitin Associated Protein 1 (UBAP1)" . ., .-..
Assembly members:
UBA domain, polymer, 63 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
UBA domain: GSSGSSGAYSELQMLSPSER
QCVETVVNMGYSYECVLRAM
KKKGENIEQILDYLFAHSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 245 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 387 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBA domain | 1 |
Entities:
Entity 1, UBA domain 63 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ALA | TYR | SER | ||||
| 2 | GLU | LEU | GLN | MET | LEU | SER | PRO | SER | GLU | ARG | ||||
| 3 | GLN | CYS | VAL | GLU | THR | VAL | VAL | ASN | MET | GLY | ||||
| 4 | TYR | SER | TYR | GLU | CYS | VAL | LEU | ARG | ALA | MET | ||||
| 5 | LYS | LYS | LYS | GLY | GLU | ASN | ILE | GLU | GLN | ILE | ||||
| 6 | LEU | ASP | TYR | LEU | PHE | ALA | HIS | SER | GLY | PRO | ||||
| 7 | SER | SER | GLY |
Samples:
sample_1: UBA domain mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.8996, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAC11162 BAC11176 BAC11224 BAC11323 BAC11342 |
| EMBL | CAB66667 CAG38582 CAL38740 |
| GB | AAF37827 AAH20950 AAH98141 AAH98316 AAH99726 |
| REF | NP_001164672 NP_001164673 NP_001164674 NP_001164675 NP_001253443 |
| SP | Q9NZ09 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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