BMRB Entry 11273
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11273
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Title: Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein
Deposition date: 2010-08-09 Original release date: 2011-08-18
Authors: Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Zhao, C.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the Novel Identified UBA-like Domain in the N-terminal of Human Fas Associated Factor 1 Protein" . ., .-..
Assembly members:
UBA-like domain, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
UBA-like domain: GSSGSSGGSAASSALKGLIQ
QFTTITGASESVGKHMLEAC
NNNLEMAVTMFLDGGGSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 211 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 334 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBA-like domain | 1 |
Entities:
Entity 1, UBA-like domain 62 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLY | SER | ALA | ||||
| 2 | ALA | SER | SER | ALA | LEU | LYS | GLY | LEU | ILE | GLN | ||||
| 3 | GLN | PHE | THR | THR | ILE | THR | GLY | ALA | SER | GLU | ||||
| 4 | SER | VAL | GLY | LYS | HIS | MET | LEU | GLU | ALA | CYS | ||||
| 5 | ASN | ASN | ASN | LEU | GLU | MET | ALA | VAL | THR | MET | ||||
| 6 | PHE | LEU | ASP | GLY | GLY | GLY | SER | GLY | PRO | SER | ||||
| 7 | SER | GLY |
Samples:
sample_1: UBA-like domain, [U-13C; U-15N], 0.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9321, Kobayashi, N. - data analysis
CYANA v1.0.8, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| GB | EDM11425 EDM11426 EDM11427 KFM11413 KFO08321 |
| REF | NP_001100556 XP_003477156 XP_004003056 XP_004644633 XP_004712317 |
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